In order to protect water resources，realize green development and gold extraction with environmental protection，it was tried to use ten pro-environment type regents for gold leaching，and selected better regents used for further industrial test.The results showed that one of the gold extraction regents had the advantages of environmental protection，fast procedure and easy operation.For example，leaching efficiency reached to70.47% for 747 g/t mineral aggregate，particularly for oxidized ore，leaching efficiency can even be up to75.16%.Thus，the pro-environment type regent was applied to CIP process for six months production.The results of practice in production demonstrated that this gold extraction regent can replace to NaCN，and leaching efficiency was about 72.92%for 650 g/t mineral aggregate，showing that the production cost reduced by 4.78 RMB/t and 0.57 million RMB/year.Obviously，this kind of gold extraction regent has the good economic benefit and remarkable environmental benefit，hence it can be applied in the gold industry in future.